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For experiments in 384-well plates, 500 cells were seeded per well, and after 24 h, the medium was switched using a series of 4 aspirationwash steps with the desired final medium using Multidrop Combi (Thermo)

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Recently, a mimetic peptide of TSP-1 called 4N1Ks has been developed as a senolytic therapy and confirm the high interest to investigate CD47 pathway in senescence 48

Depletion of Tregs in a model of fibrosarcoma led to HEV neogenesis, enabling recruitment of T-cells into the tumor [330]