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A senescence -galactosidase assay was conducted using the senescence -galactosidase staining kit (Cell Signaling Technology, 9860) according to the manufacturers instructions, and then stained cells were imaged using a ZEISS Primovert inverted microscope (Carl Zeiss Microscopy)

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2) Transfer 500 l Glutathione Magarose Beads (20% v/v) into a clean tube

The worm suspension was centrifuged at 14000 g, 4 C for 10 min and the acid supernatants transferred

10.1016/j.tibs.2017.11.004 261 SolimanH

Besides AhR and ROS, other pathways can mediate the NF-B pathway, typically such as indole uremic toxins triggering JNK/MAPK/NF-B signaling (31)