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Precast SDS-polyacrylamide gels (415% Mini-PROTEAN TGX Precast Gel) and Trans-Blot Turbo transfer system were purchased from Bio-Rad

Besides triggering a sequence of events that cause toxic outcomes, exposure to xenobiotics leads to induced oxidative stress, generating an over production of reactive oxygen species (ROS) [49] and consequently triggering oxidative damage to macromolecules such as proteins, lipids, and nucleic acids [50, 51]

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For other vial strengths the principle is the same aim for a 1 mg/ml final concentration so the math at the syringe stays straightforward