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HECT and RBR ligases form covalent thioester intermediates with ubiquitin prior to substrate transfer, whereas RING-type ligases, the most abundant class, mediate the direct transfer of ubiquitin from E2 to the substrate

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This is a meaningful safety feature that IGF-1 LR3 does not share

Garlic constituent diallyl trisulfide prevents development of poorly differentiated prostate cancer and pulmonary metastasis multiplicity in TRAMP mice

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