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Its small size is mechanically an asset for the oral route: fewer bonds to hydrolyze, less surface exposed to proteases
The easily convertible redox states Cu(I) and Cu(II) generate distinguishable bioligand variations (speciation)
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The supernatant was transferred to LCMS vials, and the pellet was lysed by 20 mM NaOH and protein levels were detected for normalization
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Once phosphorylated, HSL appears to expose more hydrophobic regions
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