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Peripheral GLP-1R is bound by GLP-1 released from intestinal secretory cells. Although small amounts of circulating GLP-1 may reach regions that lie outside the bloodbrain barrier, most brain GLP-1 signaling originates locally

Further support for the functional importance of non-helical conformations near the agonist N-terminus was obtained from a BRET-based assay that monitors receptor-mediated changes in G protein conformation

Marano, F

& Schmidt, E

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The cell lysates were centrifuged at 16,000 g and 4 C for 20 min