Gatto F, Miess H, Schulze A, Nielsen J
10.1111/ijd.17157 125 van GeelN.SpeeckaertR.LambertJ.MolletI.De KeyserS.De SchepperS.et al (2012)
Cyclic AMP assay Islets were isolated as previously described (59) and dispersed to single cell suspension using Enzyme Free Dissociation Buffer (Gibco)
Therefore, the final output reflected the average value from a mixed population of cells in different stages, that is, from cells in which the analyzed promoter might have contained bound Pol II and was transcribed, and also from cells in which the analyzed promoter might have been methylated and repressed
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Because no one can live without thyroid hormone in their body