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GTT experiments were performed twice with five or six mice per test article

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TMA is synthesized from choline by choline TMA-lyase (CutC)/activator CutD, and from dietary L-carnitine by two processes: 1) One-step conversation of L-carnitine to TMA by carnitine oxygenase/reductase (CntA/B) and 2) by two-step pathway that involves -butyrobetaine (BB)

This effect was not sensitive to naloxone, indicating a distinct mechanism from its opioid-mediated antinociceptive effects 8

Waseem Al-Zamil, MD

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