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Using an ERE-luciferase mouse, a reporter mouse that allows monitoring of ER transcriptional activity in a spatiotemporal manner, it was demonstrated that estrogen signaling is locally activated in keratinocytes in vitro (with 1 nM E2) and in vivo (with 10 nM E2)

The commenter stated that even though CMS finalized a 4-year phased transition of the SA058 pricing to soften the impact, the cut remains severe and that CMS should ensure the updated supply pack and equipment times accurately reflect true office costs