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NIH/3T3 cells were seeded on 6-well plates (2 10 4 cells per well) and cultured for 24 h

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Test tubes and Eppendorf tubes to prepare standards and samples

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Yamaura K, Ogawa K, Yonekawa T, Nakamura T, Yano S, Ueno K

Case report 1 Melanosis coli associated with chronic laxative abuse Patient information Age: 55 Gender: Female Medical history: Hypertension, irritable bowel syndrome Chief complaint: Chronic constipation and abdominal discomfort Duration of symptoms: 3 years Summary A 55-year-old female presented with a history of chronic constipation and abdominal discomfort for the past 3 years