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The lysates were collected by scraping and then subjected to sonication for 1 min

Volatile organic compounds (VOC) analysis To measure the temporal production of VOCs in the bottles, different incubation times were tested for each culture condition: 48, 72 and 96 hours at 37 C

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This makes them water-soluble and able to be excreted through urine or feces.[ref] Essentially, these two steps need to work in tandem: Phase I : The CYP1B1 or CYP1A1 enzyme breaks down estradiol into the catechol estrogen metabolites

Acknowledgements Amgen sponsored this study and was involved in the study design

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