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Blum WF, Crowe BJ, Quigley CA, et al

This mutation also results in a CPT1A protein that is less sensitive for the inhibitory effect of malonyl-CoA on CPT1A under fed conditions, resulting in a 34 times higher activity of CPT1A compared to wild type [91,92,93]

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Cell line culture GLUTag cells (a kind gift from Dan Drucker, Toronto) were maintained in Matrigel-coated flasks and low-glucose DMEM supplemented with 10% FBS, 100 units ml 1 penicillin and 0.1 mg ml 1 streptomycin