Specifically, upon GSK-3 phosphorylation Nrf2 can bind to -TrCP, another ubiquitin ligase that is independent of the redox status of the cell, but sensitive to cell cycle regulation and nutrient sensing [16]
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The GGT-shRNA-Lentivirus was diluted in DMEM with 10% FBS and supplemented with polybrene at a final concentration of 5 g/mL to enhance transduction efficiency
8:515-525 [PubMed: 22432790] 414
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