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Media was removed and cells were fixed with 3.7% formalin in phosphate buffered saline and immunofluorescent labeling performed for E-Cadherin (#3195T;Cell Signaling Technology Inc.)

10.1093/humrep/dem300 15 De CelisR.Feria-VelascoA.Gonzalez-UnzagaM.Torres-CallejaJ.Pedron-NuevoN

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These CVOT findings are supported by a real-world study performed in over 3 million patients with T2DM from the United States, in which patients treated with SGLT2is did not have a higher risk of any or lower amputation compared with incretins or other glucose-lowering agents [77]

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Experts say this large cohort study strengthens the case for using these GLP-1 medications in people with this heart condition