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These included glucose and insulin tolerance tests to define whole-body glucose homeostasis, 13 C-isotope tracing in conscious unrestrained mice to determine the tissue-specific fate of glucose and serine in vivo , and metabolomics to quantify metabolite concentrations

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2) Functionalization of the catalyst

Data was analyzed using the relative quantitative method, by normalizing the mRNA level of all genes against that of -actin gene

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For the western blot analysis, we used each of two custom antibodies on both whole aphid bodies and on dissected bacteriocytes