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Lysosomes and ER communicate through membrane contact sites, facilitating lipid transfer

These studies underscore the paradoxical role of arginine metabolism and availability depending on the circumstances (e.g., immune cells infiltration and the type of arginine metabolizing enzymes expressed in these cells in the TME) and type of tumor, implying that arginine supplementation might be interesting for some, but not other tumors (Table 3)

The experiments with isolated mitochondria (0.5 mg/mL) were carried out at 28C with a standard reaction medium containing 15 M Ca 2+ , 10 mM Hepes, 125 mM sucrose, 2 mM K 2 HPO 4 , 65 mM KCl, 1 mM MgCl 2 , and 5 mM glutamate plus 5 mM malate (pH 7.2)

Proceedings of the National Academy of Sciences, 81(5), 13271331

Ca 2 + -influx Measurements Ca + 2 -influx Measurements Calcium influx in FLS after TRPA1 opening was observed by calcium imaging as in our previous study [24]