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Secondary analysis using an ELISA based approach confirms GPx3 is substantially reduced in children affected with konzo To validate the findings obtained using proteomic-based applications, we sought to determine if the reduced abundance of GPx3 protein in konzo affected children as compared to their unaffected siblings could also be detected using an orthogonal ELISA-based approach

After extrusion the liposomes were kept for 20 min at room temperature, then at 4 C for 1 hour and finally on ice

Reagent 2

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