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The mRNA levels for modulated genes were determined by reverse transcription of total RNA followed by quantitative real-time PCR analysis (qRT-PCR) on a Quant Studio 6 Flex system (Applied Biosystems, Life Technologies, U.S.) using SYBR PCR Master Mix reagent kits (Takara, Japan) following the manufacturers protocol

The right choice depends on the pathway being studiednot on the assumption that four peptides must be better than three

H., Golubeva, A