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Mushroom tyrosinase inhibition assay To evaluate the inhibition of the mh-Tyr by the selected flavonoids, i.e., C3G, EC, and CH, against positive control, i.e., ARB inhibitor, two different approaches, including in vitro mh-Tyr inhibition using spectrophotometer method and visual examination of enzyme inhibition by zymography method, were used to monitor the mh-Tyr activity under different concentrations of the respective compounds (Table S4)

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In conclusion, the findings of this study indicate that all examined biomarkersinterleukin-6 (IL-6), C-reactive protein (CRP), and tumor necrosis factor-alpha (TNF-)may serve as valuable prognostic indicators for potential complications associated with diabetes

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