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Isolation and culture of pancreatic islets Mice were sacrificed by cervical dislocation and the pancreas was excised after infusing the animal in the common bile duct with 34 mL of Collagenase P solution (13.5 U/mL) derived from Clostridium histolyticum mixed with HBSS (1:14) following the procedure reported by Carter et al

The cell pellets were then washed twice with 1X phosphate buffer (0.1 M, pH 7.4)