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A typical brewed green tea beverage, consisting of 25 g tea leaves in 250 ml of hot water, contains 2050 mg caffeine (Reference Balentine, Wiseman and Bouwens49,Reference Sang, Lambert and Ho50) , compared with 235 mg caffeine in 12 fluid oz

Each sample mixed with light (endogenous) and heavy (synthetic) peptides was quantified by integrating the quantifier fragment ions to derive a light-to-heavy peptide ratio

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