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Forgetting to wipe the stoppers, touching the needle, or working on a dirty surface can introduce bacteria
Anal Biochem 423(2):236240 Sabiu S, Garuba T, Sunmonu T, Ajani E, Sulyman A, Nurain I et al (2015) Indomethacin-induced gastric ulceration in rats: protective roles of Spondias mombin and Ficus exasperata
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Next, the secondary antibody, which was conjugated to HRP, was diluted in the blocking buffer and incubated with the membrane for 1 h at room temperature