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10, 2022 from Sepah, Y., Samad, L., Altaf, A., et al

Do not keep liquid items above the pens to avoid accidental spills

View rest periods as part of the program, not interruptions to it

Another dCas9 fusion is the synergistic activation mediator (SAM) system, which consists of three total components: dCas9 fused to VP64 with a nuclear localization sequence (NLS) (NLS-dCas9-VP64), two activation domains (p65 and human heat-shock factor 1 (HSF1)) fused to the MS2 bacteriophage coat protein (MS2-p65-HSF1), and the sgRNA fused to two MS2 protein-targeting hairpin aptamers at the stem-loop 2 and tetraloop regions (sgRNA2.0) [70]

Cooper, G