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Early work, including in the Journal of Medicinal Chemistry, has mapped these relationships to guide the design of next-generation NNMT inhibitors

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The sequence parameters included a TE of 80 ms, TR of 2,000 ms, water suppression using a multiple optimized insensitive suppression train, pulse duration of 20 ms, an estimated water peak of 4.68 ppm, editing pulses interleaved at 1.9 ppm (ON) and 1.5 ppm (OFF), averaging two signals, a phase cycle of 16, frequency stabilization, and a total of 160 dynamic scans