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89 , e0018000125 (2025)

mBio 9 , e0166801618 (2018)

Cytosolic and nuclear NAD + are often considered a shared pool as NAD + diffuses through nuclear pores 58 , and importantly, the concentrations of NAD + in the cytosol, nucleus, and mitochondria mirror the K m of NAD + -consuming enzymes localized to the corresponding compartment, hence these enzymes are sensitive to compartmental fluctuations in NAD + levels 77

In addition, two free -carboxyl groups of exogenous GSH can protonate with increasing acidity in the extracellular environment (e.g., culture media), which may also be associated with antibacterial activity