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The direct binding of insulin-like growth factor-1 (IGF-I) to integrin alphavbeta3 is involved in IGF-I signaling

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Temperature shock can cause precipitation, where the peptide crashes out of solution and forms visible particles that indicate permanent damage

Reconstituted with 2 mL bacteriostatic water for a 5,000 mcg/mL total-blend concentration (2,500 mcg/mL per peptide)

The resulting fractions were then re-suspended in buffer A (16 L) and analyzed on an Orbitrap Fusion mass spectrometer

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