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That is the minimum

doi: 10.1038/s41422-020-00441-1

WT samples for each peptide-spectra match by dividing each TMT reporter ion intensity by the average intensity for the channels corresponding to WT control (3 per TMT sample)

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If using a PPI, the morning dose provides all-day coverage, but adding an evening H2 blocker specifically for nighttime protection is a well-established combination strategy