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Currently, there is no direct evidence that GLP-1RA use contributes to progression of pancreatic cysts to PDAC, but this remains an important knowledge gap for future investigations considering the high prevalence of pancreatic cysts in the general population (~ 10% by age 50 and ~25% by age 70) 56

It's used for basic or hydrophobic compounds that won't dissolve at neutral pH

After 28 days, the cumulative risk of contamination from repeated entries and the potential for gradual degradation of the benzyl alcohol become too significant to ignore

Dimethyl sulfoxide diluted in a .01 M glycine buffer was added to solubilize the cell membranes and the absorbance of reduced MTT in the buffer was quantitated at 590 nm using a plate reader (Biotek Synergy 2, Winooski, VT)