Gene expression analysis via qRT-PCR Total RNA was isolated from 50 to 100 mg of liver tissue using the RNeasy Mini kit (Qiagen, Tokyo, Japan), followed by reverse transcription using a high-capacity RNA-to-cDNA kit (Thermo Fisher Scientific, Tokyo, Japan) according to the manufacturers instructions
Improvement in lipid profile (reduction in triglycerides and LDL cholesterol)
Rosenstock also noted that results from the EMPA-REG trial of empagliflozin (Jardiance, Boehringer Ingelheim) were a real game changer. It is the only drug that has an indication for reduction of cardiovascular death
Pharmaceutical companies are rebranding GLP-1 therapies for dual purposes, targeting lifestyle-driven consumer segments
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Concentrations of 10-20mg/ml work well, requiring 0.05-0.3ml injection volumes that standard insulin syringes measure accurately