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It has FDA approval history for specific pediatric indications and has long been used in certain clinical settings where healthcare providers evaluate growth hormone-related concerns in adults

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The observed differences in free GSH, GSSG, and their respective ratio is likely due to spontaneous oxidation of free GSH in the heparinized plasma rather than a matrix effect of anticoagulant type used during sample collection

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Samples were then incubated at -20 C for 30 min before centrifugation at 12,000 rpm for 10 min at 4 C, discarding the pellet