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Wild type -syn overexpression or mutation causes mitochondrial fragmentation, mitochondrial membrane potential reduction, mitochondrial axonal transport inhibition, reactive oxygen species (ROS) production, and Ca 2+ homeostasis imbalance at mitochondria-associated membranes (MAMs), see Melo et al.s (2018) review

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The menstrual cycle phase was determined for women at the time of the study to avoid possible confounding effects on anxiety and depression symptoms

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The nanoprobes, i.e., BEAGA/ApoE-PDHD@ILD & R848, ApoE-PDHD@ILD & R848, and BEAGA-PDHD@ILD & R848, were added into the medium of minicells and incubated at 37 C

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