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UVvis spectra were acquired using a Multiskan SkyHigh microplate spectrophotometer (ThermoFisher Scientific) and 96-well plates (Corning) or quartz cuvettes using SkanIt software

This "bottom-up" approach works well for many people, especially those with healthy liver function and the necessary enzymes to complete the conversion

This preparation contains a total peptide content exceeding the labelled amount based on independent analytical verification

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Plasmid construction and siRNA Plvsin-GGCT-eGFP expression plasmid was constructed by inserting the full-length GGCT sequence amplified from human cDNA and ligated into the Plvsin-eGFP vectors