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These shots are delivered directly into the bloodstream which bypasses the gastrointestinal system and ensures that the body gets a sufficient amount of vitamin B12 stores to function optimally

Material and methods Cell culture Human hepatocellular carcinoma (HepG2) (ATCC, HB-8065) were maintained in DMEM (4.5 g/l glucose, pyruvate) (Gibco), supplemented with 10% fetal bovine serum (PAA Laboratories) and penicillin/streptomycin (25 g/ml each, Gibco) at 37 C, 5% CO 2

2010 Jan 12;3(1):125-145