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Activity of ADA isoforms (ADA 1 and ADA 2) To estimate the ADA activity of isoenzymes, we have taken the aliquots of the same samples for which the metabolomic analysis, and cytokine assays were done previously

Fluorescence/luminescence/absorbance intensities at appropriate wavelengths were quantified in Synergy H1 microplate reader (version 2.09.1)

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Conversely, daily TZP bolus induced mild hepatocellular vacuolation and perivascular inflammation, which was partially mitigated in the PNP-1-10-0.05 TZP (4.5 mg/mL) formulation group