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Integr Cancer Sci Ther

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Binding of cAMP reduces FRET so that when exciting with 440 nm light, there is an increase of 485 nm (mTuquoise2) and a decrease of 535 nm (cp173 Venus-Venus) emission fluorescence

Science (2018) 359(6382):140811

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Regarding the former, unlike single-antigen vaccines such as peptide-based formulations, whole-cell vaccines retain the full antigenic repertoire of tumor cells, enabling simultaneous activation of polyclonal T cell responses against multiple antigens and thereby reducing the likelihood of immune escape