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Both monothiol roGFP2 variants were rapidly glutathionylated with residue C208 reacting about twice as fast as residue C151 regardless whether PfGrx C32S/C88S (SSG) or PfGrx C88S (SSG) was used as the oxidant
Journal of Alternative and Complementary Medicine, 17 (5), 427-434
The cell releases its stored contents: histamine, tryptase, prostaglandins, leukotrienes, and a cocktail of other inflammatory mediators
Cell lines and culture conditions HepG2 cells, a human hepatoma cell line, were obtained from the American Type Culture Collection (Manassas, VA) and were cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% (v/v) fetal bovine serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere