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ROS-mediated cell signaling As outlined above, ROS have origin from two main sources: mitochondrial oxidative metabolism and processes of cellular response to xenobiotics, bacteria, and cytokines during which ROS are formed as a part of either signal transduction pathways or a cellular defense mechanism (Finkel 2011)

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DEER uses dipolar coupling between two SL, often tethered to the surface of the same protein, to measure the distance between these SL