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To determine the amount of NFT required after its initial addition and a washout phase to maintain elevated glutathione levels, cells were pretreated with 20 M NFT for 48 h, maintained in NFT-free medium for 24 h, and subsequently treated with varying concentrations of NFT over 24 h

10.1101/gad.275891.115 43 JiH.WangW.LiX.HanX.ZhangX.WangJ.et al (2022)

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That said, "generally safe" does not mean "universally safe for everyone in all circumstances." Individual reactions vary