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They concluded that while GHK-Cu does demonstrate anti-wrinkle effects in cellular studies, "there is a surprising absence of clinical studies using them." The review identified skin permeation as the central challenge: GHK's high aqueous solubility and low partition coefficient work against transdermal absorption

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The scheduled MRM window was 60 sec, optimized oxylipin parameters were adopted 115 , and the curtain gas pressure was set to 35 psi