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The cell pellet was suspended in 10% ice-cold TCA and centrifuged for 15 min at 9,000 g

This direct approach allows for immediate utilization by the body, leading to faster and more noticeable results compared to oral supplements

doi: 10.1038/ismej.2014.99 221 RadAHAbbasalizadehSVazifekhahSAbbasalizadehFHassanalilouTBastaniPet al

doi: 10.1515/dmdi-2020-0119 62 EmilianiFESedlakTWSawaA

Impairment of short-chain fatty acid (SCFA) and l-isoleucine biosynthesis in gut microbiome persists beyond 30 days after recovery from COVID-19, which could contribute to persistent leaky gut and dysbiosis in PASC patients 237,238

Videos were recorded at 150 frames per segment under consistent illumination and magnification for each experimental condition