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For genotyping of the TP53 P72R SNP (rs1042522) 1550 ng of DNA was amplified using M13 tailed primers spanning exon 4 (TP53_ex4_F: tgagtggatccattggaaggg, TP53_ex4_R: tccaaacaaaagaaatgcagggg) and the HotstarTaq PCR Mastermix (5 units/l) (QIAGEN, Hilden, Germany)

Before your session, stay hydrated and avoid heavy meals

Working Strength Conjugate was then added (75 L/well) and incubated for 2 h at room temperature on a microplate shaker set to 750 RPM

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doi: 10.1016/S0140-6736(18)30533-6 124 EigentlerTKHasselJCBerkingCAberleJBachmannOGrunwaldVet al

Regarding conversion to AA, there seems to be no data about it, so we can neither deny nor confirm it