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After each incubation, the supernatant was collected and transferred into a separate tube

However, repeated room temperature exposure has a cumulative effect

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Anatomical images were acquired using a T 2 -Turbo-RARE scan, TR = 2230 ms, TE = 39.43 ms, FOV = 20 7.9 mm 2 , slices: 23, slice thickness = 0.3 mm, matrix size = 200 79

We examine the role of receptor binding kinetics and -arrestin-biased signaling in the observed trafficking profiles, identifying a linked set of agonist characteristics optimally suited for insulin secretion, not shared by GLP-1R agonists in the current clinical use

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