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Protein digestion by trypsin was performed according to the filter-aided sample preparation (FASP) procedure described by Matthias Mann ( Figure 1 2.6 LC-MS/MS analysis Labeled peptides were fractionated by High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific)

[DOI] [PubMed] [Google Scholar] 15.Jiskoot W., Randolph T.W., Volkin D.B., Middaugh C.R., Schneich C., Winter G., Friess W., Crommelin D.J., Carpenter J.F