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At 48 h following transfection HepG2 cells were treated with 0.1 mL DMEM without FBS containing 50 M AQ-QI or 30 M DEAQ-QI for 16 h

The dosing schedule began at 14 mg in week 1, followed by 28 mg (week 2), 42 mg (week 3), and 56 mg for five consecutive weekly administrations (week 4, 5, 6, 7, 8)

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GST pull-down was mixed with cell lysates from HEK293T cells, washed, and immunoblotted for NMHC-IIA and GST

Molecular Therapy, 25(7), 1628-1640