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Here is a detailed examination of these challenges and the corresponding solutions: Tag-Induced Protein Aggregation Challenge : One of the primary issues with GST-tagged proteins is that the GST tag can sometimes induce aggregation or interfere with the folding of the target protein

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In addition, not all the Bacillus strains tested contained the aiiA gene, suggesting that other enzymes may be responsible for the observed hydrolysis of AFB1