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After being washed in PBS, the slides were stained with hematoxylin and eosin, mounted with Canada balsam (03984, Sigma-Aldrich), and observed with an epifluorescence microscope (IX71, Olympus, Tokyo, Japan) equipped with a digital imaging system (DP71, Olympus)

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The 50mg format is designed for extended or multi-phase laboratory programs investigating cellular senescence and longevity mechanisms