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Each peptide sample was resuspended in 80 l of 50 mM HEPES and labeled with 20 l of the 25 g l 1 TMT reagents in acetonitrile

Biohacker Protocol (experimental) Dose: 0.05 0.1 mg (= 24 clicks) Duration: 4 6 weeks Frequency: Daily microdose before rest cycle Cycle Interval: 1 2 weeks off before repeating Goal / Description: Low-dose continuous exploration of sleep quality and recovery markers

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16 Methods Radiochemistry The radiolabelling synthesis and quality control of [ 68 Ga]Ga-DO3A-Exendin-4 was conducted according to the method published earlier