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There was also a decrease in the viability of M

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The tissue was then blotted dry and weighed followed by homogenization in 200 l buffer (0.05 M potassium phosphate and 0.1 mM EDTA, pH 7.8) and centrifuged at 15,000 xg for 30 min at 4C

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The clinical application of these markers in preoperative risk stratification, guiding perioperative immunonutrition, and dynamic monitoring for early complication detection is thoroughly discussed

DNA was extracted using the AutoGen (Holliston, MA) QuickGene-610L nucleic acid extraction machine with the Fuji QuickGene DNA Whole Blood Kit (AutoGen), following manufacturers instructions