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The medium was changed to a serum-free medium (D6046, Sigma-Aldrich) 48 h after seeding, and sodium selenite (100 nM, S9133, Sigma-Aldrich) was added at the same time for maintaining the expression of GPX3

Test compounds were administered via oral gavage to all mice groups after 1 hour of fasting

Therefore, specific topical inhibitory peptides could be designed to selectively inhibit LH receptors in hair follicles to treat FPHL [142,143]

Michigan, Rhode Island and Wisconsin are planning or considering restrictions, according to KFFs most recent survey