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The resulting lysate was clarified by centrifugation at 19,000 g for 20 min at 4 C

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The two routes and compartments do not overlap, so co-ingestion timing is irrelevant from an absorption standpoint

Firstly, we detected the expression and location of FOXO4 in mice testes by immunohistochemical staining and found that FOXO4 was rarely expressed in young mice testes, but expressed typically in the Leydig cells of aged mice (Figure 6A)

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